Question 1

The bending of light rays as they pass from one medium to another is called refraction.

Accepted Answer

A) True 
 B)False

Question 2

Which of the following puts the Six "I"s in their correct order?

Accepted Answer

A)  inoculation, incubation, isolation, inspection, identification
B)  isolation, inspection, inoculation, incubation, identification
C)  incubation, inspection, isolation, identification, inoculation
D)  inspection, identification, isolation, incubation, inoculation
E)  inspection, isolation, incubation, inoculation, identificationF) D) and E)
G) A) and D)

Question 3

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?

Accepted Answer

A)  NaCl
B)  sheep red blood cells
C)  bile salts
D)  thioglycolic acid
E)  peptoneF) A) and B)
G) A) and C)

Question 4

This microscope does not use light in forming the specimen image:

Accepted Answer

A)  bright-field
B)  dark-field
C)  phase-contrast
D)  fluorescence
E)  electronF) B) and D)
G) C) and D)

Question 5

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the _____.

Accepted Answer

The answer of The correct microbiological term for the tiny...

Question 6

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A)  differential
B)  selective
C)  enumeration
D)  enriched
E)  reducingF) B) and D)
G) C) and E)

Question 7

In lab, Tom was given a mixed culture.His objective is to isolate single colonies.What should be used to accomplish this goal?

Accepted Answer

A)  Sterile swab, loop dilution, TSB
B)  Hockey stick, spread plate technique, and 4 degree Celsius incubation temperature
C)  Loop dilution, TSA, hockey stick, 37 degree Celsius incubator
D)  Inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator
E)  Sterile swab, streak plate method, 37 degree Celsius incubatorF) A) and D)
G) A) and C)

Question 8

Compare and contrast the reagents and functions of negative staining versus positive staining.

Accepted Answer

Negative staining and positive staining ...

Question 9

The wavelength of light used plus the numerical aperture governs

Accepted Answer

A)  illumination.
B)  resolution.
C)  magnification.
D)  size of the field.
E)  All of the choices are correct.F) B) and E)
G) B) and D)

Question 10

The three physical forms of laboratory media are: solid, semisolid, and _____.

Accepted Answer

The answer of The three physical forms of laboratory media...

Question 11

All of the following are examples of basic dyes except

Accepted Answer

A)  methylene blue.
B)  nigrosin.
C)  crystal violet.
D)  safranin.
E)  fuchsin.F) B) and E)
G) C) and E)

Question 12

A microbiologist decides to use a nutrient medium that contains thioglycolic acid.What type of microbe is she attempting to culture?

Accepted Answer

A)  fastidious
B)  gram positive
C)  anaerobe
D)  gram negative
E)  virusF) C) and E)
G) A) and C)

Question 13

Which type of media can be used to determine if a bacteria is motile?

Accepted Answer

A)  SIM
B)  MacConkey
C)  Enriched media
D)  Thayer-Martin media
E)  Chocolate agarF) A) and C)
G) A) and B)

Question 14

This microscope achieves the greatest resolution and highest magnification:

Accepted Answer

A)  bright-field
B)  dark-field
C)  phase-contrast
D)  fluorescence
E)  electronF) D) and E)
G) A) and E)

Question 15

A pure culture contains only

Accepted Answer

A)  one species of microorganism.
B)  bacteria.
C)  a variety of microbes from one source.
D)  None of the choices are correct.E) A) and D)
F) B) and C)

Question 16

All of the following are correct about agar except

Accepted Answer

A)  it is flexible.
B)  it melts at the boiling point of water (100°
C)  .
C)  it is a source of nutrition for bacteria.
D)  it solidifies below 42°C.
E)  it is solid at room temperature.F) A) and D)
G) B) and C)

Question 17

The Gram stain, acid-fast stain, and endospore stain have the following in common:

Accepted Answer

A)  used on a wet mount of the specimen
B)  use heat to force the dye into cell structures
C)  outcome based on cell wall differences
D)  use a negative stain technique
E)  are differential stainsF) B) and E)
G) A) and B)

Question 18

Which of the following magnifies the specimen to produce the real image of the specimen?

Accepted Answer

A)  condenser
B)  objective lens
C)  ocular lens
D)  body
E)  nosepieceF) A) and B)
G) All of the above

Question 19

This microscope shows cells against a bright background and also shows intracellular structures of unstained cells based on their varying densities:

Accepted Answer

A)  bright-field
B)  dark-field
C)  phase-contrast
D)  fluorescence
E)  electronF) None of the above
G) C) and D)

Question 20

Explain how and why immersion oil increases resolution but not magnification when using the 100X objective.

Accepted Answer

Immersion oil increases resolution but n...

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

Which of the following puts the Six "I"s in their correct order?

Correct AnswerverifiedShow Answer

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?

Correct AnswerverifiedShow Answer

All of the following are examples of basic dyes except

Correct AnswerverifiedShow Answer

A pure culture contains only

Correct AnswerverifiedShow Answer

This microscope achieves the greatest resolution and highest magnification:

Correct AnswerverifiedShow Answer

Compare and contrast the reagents and functions of negative staining versus positive staining.

Correct AnswerAnswered by ExamLex AINegative staining and positive staining ...View AnswerShow Answer

Explain how and why immersion oil increases resolution but not magnification when using the 100X objective.

Correct AnswerAnswered by ExamLex AIImmersion oil increases resolution but n...View AnswerShow Answer

This microscope shows cells against a bright background and also shows intracellular structures of unstained cells based on their varying densities:

Correct AnswerverifiedShow Answer

Which of the following magnifies the specimen to produce the real image of the specimen?

Correct AnswerverifiedShow Answer

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Correct AnswerverifiedShow Answer

In lab, Tom was given a mixed culture.His objective is to isolate single colonies.What should be used to accomplish this goal?

Correct AnswerverifiedShow Answer

The wavelength of light used plus the numerical aperture governs

Correct AnswerverifiedShow Answer

All of the following are correct about agar except

Correct AnswerverifiedShow Answer

This microscope does not use light in forming the specimen image:

Correct AnswerverifiedShow Answer

Which type of media can be used to determine if a bacteria is motile?

Correct AnswerverifiedShow Answer

A microbiologist decides to use a nutrient medium that contains thioglycolic acid.What type of microbe is she attempting to culture?

Correct AnswerverifiedShow Answer

The three physical forms of laboratory media are: solid, semisolid, and _____.

Correct AnswerverifiedShow Answer

The Gram stain, acid-fast stain, and endospore stain have the following in common:

Correct AnswerverifiedShow Answer

The bending of light rays as they pass from one medium to another is called refraction.

Correct AnswerverifiedShow Answer

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the _____.

Correct AnswerverifiedShow Answer

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Negative staining and positive staining ...
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Immersion oil increases resolution but n...
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